when i said lab was depressing on the last post, well i was hoping that it will get better. but it ended up worse.
my bacterial stock cultures that i kept in the freezer for further tests and also as a backup incase the ones in the plates dies off seemed to have some problems.
when i tried sub culturing it (reviving it back) into a new fresh broth last 2 days ago, half of it shows no growth. i tried adding more again yesterday, and today that half of it shows no growth at all.
the other half that could grow suddenly didn't show any signs of growth after i sub cultured it for the second time. i did the second time to ensure that it will be active and all..but why no growth?
it's already near the end of the experiement and my bacterias showing no growth? i was praying and hoping that its not dead like how my SV said it might be..
with my friend not talking to me, possibility of bacteria dying, group mate's being an ass, lab session could not get any more depressing.
i was on the edge, i was thinking of throwing everything away, go back home, get new wheatberries, calm down, get back to uni and start all over again.
it's like the wheatberries crisis all over again. i guess i need to be more patient..
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